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Microbial Cell Surface Analysis. Structural and Physicochemical Methods. During recent years, this method has found application in the study of the surface characteristics of a wide variety of bacteria and bacterial mixtures

Microbial Cell Surface Analysis. During recent years, this method has found application in the study of the surface characteristics of a wide variety of bacteria and bacterial mixtures. Correlations have been found between the adherence of bacteria to hydrocarbons and their attachment to other surfaces, including non-wettable plastics, epithelial cells, and teeth. A slight modification of the assay enables the isolation of nonhydrophobic mutants.

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Microbial Cell Surface Analysis book. Goodreads helps you keep track of books you want to read. Start by marking Microbial Cell Surface Analysis: Structural And Physicochemical Methods as Want to Read: Want to Read savin. ant to Read.

Cell surface analysis often requires manipulation of cells prior to examination. All of the cell manipulation procedures severely modified the physicochemical properties of cells, but with each procedure some organisms were more susceptible than others. The most commonly employed procedures are centrifugation at different speeds, changes of media during washing or final resuspension, desiccation (either air drying for contact angle measurements or freeze-drying for sensitive spectroscopic analysis, such as X-ray photoelectron spectroscopy), and contact with hydrocarbon (hydrophobicity assays).

Physico Chemical Analysis Method. Instrumental analysis should be the last step of EDA, enabling identification of the substances of interest, mostly using mass spectrometry and/or nuclear magnetic resonance spectroscopy combined with computer-aided tools (Figure 1. ). From: Instrumental Thin-Layer Chromatography, 2015.

Microbial cells sense and respond to their environment using their surface constituents. With its ability to observe living microbial cells at nanometer resolution and to manipulate single-cell surface molecules, atomic force microscopy (AFM) has emerged as a powerful tool in microbiology. Here, we survey major breakthroughs made in cell surface microbiology using AFM techniques, emphasizing the most recent structural and functional insights.

Physicochemical and structural studies onAcinetobacter calcoaceticus RAG-1 and . In: Mozes N, Handley PS, Busscher HJ, Rouxhet PG (eds) Microbial cell surface analysis-structural and physicochemical methods. New York: VCH Publishers, In. pp 317–338Google Scholar.

Physicochemical and structural studies onAcinetobacter calcoaceticus RAG-1 and MR-481-Two standard strains in hydrophobicity tests. Authors and affiliations.

X-ray photoelectron spectroscopy (XPS) on microbial cell surfaces requires freeze-drying of cells, and as a result, the cell surface appendages . Micro - bial Cell Surface Analysisand Physicochemical Methods.

X-ray photoelectron spectroscopy (XPS) on microbial cell surfaces requires freeze-drying of cells, and as a result, the cell surface appendages flatten out on the cell surface and form a collapsed fibrillar mass. At present, it is unclear how the density, length and composition of these fibrils influence the elemental surface composition as probed by XPS. The sampling depth of XPS can be varied by changing the electron take-off angle.

Microbial cell surface analysis: structural and physicochemical methods; VCH Publishers, In. New York, 1991. 17) Beveridge, T. Schultze-Lam, . Thompson, J. B. In Metal Speciation and Contamination of Soil; . Bowers, Ed. Lewis Publishers: Boca Raton, 1995; p 358.


Microbial Cell Surface Analysis : Structural and Physicochemical Methods download epub
ISBN: 0895737833
Category: No category
Language: English
Publisher: VCH Publishers (1991)
Pages: 368 pages