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RNA Interference: Methods for Plants and Animals (Plant Science / Horticulture) download epub

by T. Doran,C. Helliwell


Epub Book: 1302 kb. | Fb2 Book: 1946 kb.

RNA interference (RNAi) has been one of the most exciting and significant new methodologies to appear in the past decade, and it now finds widespread application. This methods manual provides an introduction to the phenomenon to RNA Interference and specific protocols for RNAi, in organisms from plants and . legans to Drosophila and mammals.

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Movement of RNA between plant host and invading pathogen represents an important phase of RNAi-mediated HIGS . A few studies show involvement of transporters in RNA uptake in animal cells

Movement of RNA between plant host and invading pathogen represents an important phase of RNAi-mediated HIGS and while little is known about the mechanism of cross-species RNA transport, this aspect of HIGS is likely to be of importance in control of toxigenic fungi. A few studies show involvement of transporters in RNA uptake in animal cells. In Caenorhabditis elegans the transmembrane protein SID-1, when expressed in Drosophila S2 cells (lacks a sid-1 homolog) enables passive dsRNA uptake from the culture medium (Shih and Hunter, 2011).

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Historically, RNAi was known by other names, including co-suppression, gene silencing (PTGS), and quelling. The detailed study of each of these seemingly different processes elucidated that the identity of these phenomena were all actually RNAi.

Plant science worksheets that cover topics in Plant Science along with well written guided notes that could help outline a unit for a new teacher.

In Communication in Plants – Neuronal Aspects of Plant Life, F. Baluska, S. .Pharmacological survey of medicinal plants for activity at dopamine receptor subtypes. Mancuso, and D. Volkmann, eds. pp. 19–35. In Melatonin and the Pineal Gland: From Basic Science to Clinical Applications, Y. Touitou, J. Arendt, and P. Pevet, eds. 83–186. Amsterdam: Excerpta Medica. and Gupta, R (2007) Acetylcholine causes rooting in leaf explants of in vitro raised tomato (Lycopersicon esculentum Miller) seedlings. Life Sci. 80(24–25): 2393–2396. PubMedGoogle Scholar.

Development of methods for RNA interference in the sheep gastrointestinal parasite, Trichostrongylus colubriformis. In RNA Interference: Methods for Plants and Animals (eds. Doran, T. & Helliwell, ., pp. 85–113. CAB International Wallingford, UK. Kamath, R. S. and Ahringer, J. (2003). International Journal for Parasitology 35, 935–940. Johnson, N. Behm, C. A. and Trowell, S. C. (2005).

Doran and C. Helliwell, RNA Interference: Methods for Plants and Animals, CABI, Oxfordshire, UK, 2009

Doran and C. Helliwell, RNA Interference: Methods for Plants and Animals, CABI, Oxfordshire, UK, 2009.

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The Principles & Protocols Series is designed for upper level undergraduates, postgraduates and researchers, particularly those new to a field. Books in the series outline the theoretical background to experimental approaches, followed by bullet-point protocols, which are generic and can be adapted for particular systems. RNA interference (RNAi) has been one of the most exciting and significant new methodologies to appear in the past decade, and it now finds widespread application. This methods manual provides an introduction to the phenomenon to RNA Interference and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. Also included are chapters covering small hairpin RNAs and viral-induced gene silencing.
RNA Interference: Methods for Plants and Animals (Plant Science / Horticulture) download epub
Biological Sciences
Author: T. Doran,C. Helliwell
ISBN: 1845934105
Category: Science & Math
Subcategory: Biological Sciences
Language: English
Publisher: CABI; First edition (December 17, 2008)
Pages: 272 pages