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Human Embryonic Stem Cells: The Practical Handbook download epub

by Stephen Sullivan,Chad A Cowan,Kevin Eggan


Epub Book: 1984 kb. | Fb2 Book: 1895 kb.

We found all the protocols tested to be robust

We found all the protocols tested to be robust. Troubleshooting sections were well written and easy to follow.

Since their isolation in 1998, human embryonic stem (ES) cells havebeen used as a powerful experimental model .

Since their isolation in 1998, human embryonic stem (ES) cells havebeen used as a powerful experimental model for studying themechanisms of stem cell self-renewal, pluripotency, anddifferentiation in humans. This book really helped us a lot. We recently started reprogrammed human skin cells via the Yamanaka method to make pluripotent stem cells and we have used the protocols in this book to characterise the cells and differentiate them into different cell types.

The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing . 5 Standard culture of human embryonic stem cells.

The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. Jeremy M. Crook, Rachel Horne, and Alan Colman.

We found all the protocols tested to be robust

We found all the protocols tested to be robust. Download (pdf, . 7 Mb) Donate Read. Epub FB2 mobi txt RTF. Converted file can differ from the original. If possible, download the file in its original format.

Unique to this book are the troubleshooting sections for human ES cell protocols. Stephen Sullivan, Chad A. Cowan and Kevin Eggan Harvard University, 2007. List of Contributors.

p. ; cm. Includes bibliographical references. Unique to this book are the troubleshooting sections for human ES cell protocols. We felt that experimental protocols, as normally published, fail to give advice on what to do when experiments fail to work, what variables are likely to be the cause, and what to change when repeating or rescuing an experiment. Please note that many contributors have multiple afliations; these are listed in full at the start of their respective chapters.

Cells: the Practical Handbook. Drs Melton, Cowan, Sullivan, Akutsu, Eggan. C and Eggan K. John Wiley & Sons, USA. ISBN: 0470033568. and Egli, begins by pointing out that hES cells culture artifacts derived from inner cell. One preferable approach for obtaining these cell lines is by deriving them from affected preimplantation genetically diagnosed embryos.

New Biological Books. Human Biology & Health. Human Embryonic Stem Cells: The Practical Handbook. Chichester (United Kingdom) and Hoboken (New Jersey): John Wiley & Sons. xx + 404 p + 5 pl; ill; index.

West Sussex, England: John Wiley & Sons Ltd. 2007. ISBN: 978-0-470033562.

Finding books BookSee BookSee - Download books for free. Stephen Sullivan, Chad A Cowan, Kevin Eggan.

With this valuable practical guide, three members of the HarvardStem Cell Institute have compiled and edited the definite handbookfor the exciting new field of human embryonic stem cell research.The editors have gathered protocols from scientists with extensivereputation and expertise, describing and comparing currently usedtechniques for the culture of human stem cells and discussing thestrengths and weaknesses of the different approaches.

Human Embryonic Stem Cells: The PracticalHandbook contains the first centralised collection ofmethods used in human embryonic stem cell biology. The book coversthe derivation of human stem cell lines, the obtaining of cellsfrom human stem cell banks, the culturing and characterisation ofthe cells, and the differentiation of the cells in vitro andin vivo. Lastly, almost all of these protocols can also beused for analyzing and manipulating induced pluripotency iPS stemcells. This allows an even greater number of opportunities forthose interested in purusing work in pluripotent stem cells,disease modelling, and other aspects of basic regenerative medicineresearch.

The novel and useful focus of this book sets it apart from otheravailable books:

Compares and evaluates the protocols used in leadinglaboratories working on human embryonic stem cellsCentred solely on practical protocols for human (not mouse)embryonic stem cell researchIncludes extensive troubleshooting sectionsAddresses the different proclivities and behaviours ofindividual human embryonic cell linesContains techniques currently known only to a small number ofspecialised laboratories worldwide

This handbook represents an essential source of up-to-datepractical information for all cell and developmental biologistsworking with human embryonic stem cells or wishing to enter thefield. It is also essential reading for clinical researchersin areas such as diabetes, cardiovascular disease, and neurologicaldiseases.

Praise from the reviews:

"...a highly readable and useful book… A notablefeature of the book is its air of openness and honesty… Thisbook… will help many to navigate the uncharted waters ofhuman embryonic stem cell biology." BRITISH SOCIETY FOR CELLBIOLOGY

"... the imaginative solutions in this book can inspire us toget past our most frustrating limitations." CELL STEM CELL

"... the richness in the details of each protocol presentedwill certainly encourage more scientists to begin studies of Humanpluripotent stem cells..."  REGENERATIVE MEDICINE

"In this fast-moving field, this [handbook] will help driveadvances of more and more researchers." DIFFERENTIATION

"...a valuable resource for seasoned and noviceresearchers... an excellent addition to the reference collection ofany medical library or research laboratory." THE AMERICANMEDICAL ASSOCIATION


Comments: (2)

Kagalkree
Excellent handbook. One chapter on endoderm induction was particularly useful for our research, as well as a section showing how different cell lines behaved in terms of differentiation potential. We found all the protocols tested to be robust. Troubleshooting sections were well written and easy to follow.
Gavinranara
This book really helped us a lot. We recently started reprogrammed human skin cells via the Yamanaka method to make pluripotent stem cells and we have used the protocols in this book to characterise the cells and differentiate them into different cell types. We are currently using Zwaka's chapter to make reporter lines. What's brilliant is there is lots in this book about what to do when things go well.
Worth every penny.
Human Embryonic Stem Cells: The Practical Handbook download epub
Biological Sciences
Author: Stephen Sullivan,Chad A Cowan,Kevin Eggan
ISBN: 0470033568
Category: Science & Math
Subcategory: Biological Sciences
Language: English
Publisher: Wiley; 1 edition (July 23, 2007)
Pages: 424 pages